Metabolites and metabolic pathways of maackiain in rats based on UPLC-Q-TOF-MS.
10.19540/j.cnki.cjcmm.20210902.201
- Author:
Wen-Lin YUAN
1
;
Zheng-Rui HUANG
2
;
Si-Jia XIAO
1
;
Ji YE
1
;
Wei-Dong ZHANG
1
;
Yun-Heng SHEN
1
Author Information
1. School of Pharmacy, Naval Medical University Shanghai 200433, China.
2. Department of Applied Chemistry, Xi'an University of Technology Xi'an 710048, China.
- Publication Type:Journal Article
- Keywords:
UPLC-Q-TOF-MS;
flavonoid;
maackiain;
metabolism in vivo;
metabolites
- MeSH:
Animals;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Metabolic Networks and Pathways;
Pterocarpans;
Rats;
Rats, Sprague-Dawley
- From:
China Journal of Chinese Materia Medica
2021;46(23):6278-6288
- CountryChina
- Language:Chinese
-
Abstract:
Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used to investigate the metabolites of maackiain in rats based on the prediction function of UNIFI data processing system and liver microsomal incubation in vitro. Ten metabolites of maackiain after oral absorption were reasonably deduced and characterized. It was found that the biotransformation of maackiain mainly included phase Ⅰ oxidation, dehydrogenation, phase Ⅱ sulfate conjugation, glucosylation conjugation, and glucuronic acid conjugation. Among them, the product of glucosylation conjugation, trifolirhizin, was identified by comparison with the reference for the first time. Liver microsomal incubation in vitro further confirmed the metabolites and metabolic pathways of maackiain in rats. The metabolites in the blood, urine, and feces complemented each other, which revealed the migration, metabolism, and excretion modes of maackiain in rats. This study lays a foundation for the further investigation of the metabolic mechanism of maackiain in vivo and the in-depth research on the mechanism of pharmacodynamics and toxicity.
