A novel pathogenic Colletotrichum species for rhizome rot disease of Polygonatum kingianum.
10.19540/j.cnki.cjcmm.20210818.101
- Author:
Wei-Si MA
1
;
Lan-Ping GUO
2
;
Xin WANG
1
;
Tie-Lin WANG
2
;
Shi-Wu YAN
1
;
Hang JIN
1
;
Zhi-Hui ZHANG
3
;
Bin YANG
1
Author Information
1. Medicinal Plants Research Institute, Yunnan Academy of Agricultural Sciences Kunming 650205, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Yunnan Kunming Blood Center Kunming 650106, China.
- Publication Type:Journal Article
- Keywords:
Colletotrichum kingianum;
Polygonatum kingianum;
phylogenetic tree;
rhizome rot disease
- MeSH:
China;
Colletotrichum/genetics*;
Phylogeny;
Polygonatum;
Rhizome
- From:
China Journal of Chinese Materia Medica
2021;46(21):5606-5613
- CountryChina
- Language:Chinese
-
Abstract:
Rhizome rot disease is one of the main disease of planted Polygonatum kingianum. In this study, six strains of pathogenic fungus was isolated from P. kingianum samples with rhizome rot disease collected from six counties in Yunnan province. Its pathogenicity was confirmed by inoculation to healthy P. kingianum rhizome according to Koch's postulates. The colonies of the isolated fungi on potato dextrose agar(PDA) were orange with abundant crescentic conidia which were eseptate with a mean size of 19. 3-24. 9 μm×5. 2-5. 9 μm and a L/W ratio of 3. 4-4. 5. There was an oil ball in the center of the conidium. It's easy to see setae on PDA colony.The phylogenetic tree based on ITS, GAPDH, CHS-1, HIS3, ACT, and TUB2 sequences by maximum likelihood(ML) method indicated that the pathogenic fungus for P. kingianum rhizome rot disease was clustered into the clade of Colletotrichum spaethianum species complex, and was close to C. spaethianum. However, there were some differences in morphological and genetic characteristics between the pathogenic fungus and C. spaethianum. Therefore, the pathogenic fungus for rhizome rot disease of P. kingianum was identified as a new Colletotrichum species named C. kingianum. The disease spreads primarily due to the plantation of infected seedlings of P. kingianum. It is necessary to choose healthy seedlings and take rigorous disinfection measures for the disease prevention.
