Rapid detection of aflatoxin B_1 in Chinese herbal medicines by indirect and direct competitive enzyme-linked immunosorbent assays: a comparative analysis.
10.19540/j.cnki.cjcmm.20210310.305
- Author:
Chang-Jian WANG
1
;
Jiao-Yang LUO
2
;
Jia-An QIN
2
;
Lei ZHANG
2
;
Shi-Hai YANG
3
;
Mei-Hua YANG
2
Author Information
1. College of Traditional Chinese Medicine, Jilin Agricultural University Changchun 130118, China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100193, China.
2. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100193, China.
3. College of Traditional Chinese Medicine, Jilin Agricultural University Changchun 130118, China.
- Publication Type:Journal Article
- Keywords:
Chinese herbal medicine;
aflatoxin B_1(AFB_1);
direct competitive enzyme-linked immunosorbent assay(dc-ELISA);
indirect competitive enzyme-linked immunosorbent assay(ic-ELISA);
rapid detection
- MeSH:
Aflatoxin B1/analysis*;
China;
Chromatography, Liquid;
Enzyme-Linked Immunosorbent Assay;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(22):5861-5866
- CountryChina
- Language:Chinese
-
Abstract:
The indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) and direct competitive enzyme-linked immunosorbent assay(dc-ELISA) were performed for the rapid detection of aflatoxin B_1(AFB_1) in Astragali Radix, Zingiberis Rhizoma Recens, Jujubae Fructus, and Nelumbinis Semen with self-made antigens and antibodies. Different extraction methods were investigated to reduce the matrix effects of different medicinal parts in Chinese herbal medicines. The sensitivity of dc-ELISA method was improved by optimizing the molar ratio of AFB_1 to horseradish peroxidase(HRP). In this study, the sensitivity(IC_(50)) of ic-ELISA and dc-ELISA was 0.046 and 0.023 ng·mL~(-1), with the limit of detection(LOD) of 0.007 and 0.004 ng·mL~(-1), respectively. The detection time was 3 h and 50 min for ic-ELISA and dc-ELISA, respectively. The recovery rates were within the range of 62.96%-104.4%, with RSDs of less than 10%. Confirmed by LC-MS/MS, three positive samples of Nelumbinis Semen were detected from 53 samples. Two ELISA methods established in this study were accurate, rapid and sensitive, and can be used for rapid screening of AFB_1 in Chinese herbal medicines such as Astragali Radix, Zingiberis Rhizoma Recens, Jujubae Fructus, and Nelumbinis Semen. In addition, the advantages and limitations of the two methods were compared and discussed, which can provide a reference for the testing institutions to choose the proper method.
