Effects and evaluation of different processing and drying methods on components in Paeoniae Radix Alba.
10.19540/j.cnki.cjcmm.20210319.303
- Author:
Qiu-Long ZHAO
1
;
Pei-Wen YANG
1
;
Da-Wei QIAN
1
;
Xiao-Kun BIAN
2
;
Yi-Fei WANG
2
;
Zhen-Hua ZHU
2
;
Sheng GUO
2
;
Tuan-Jie WANG
3
;
Hui YAN
1
;
Jin-Ao DUAN
2
Author Information
1. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/Jiangsu Provincial Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine Nanjing 210023, China.
2. National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China.
3. Kanion Pharmaceutical Co., Ltd. Lianyungang 222001, China.
- Publication Type:Journal Article
- Keywords:
OPLS-DA;
PLS-DA;
Paeoniae Radix Alba;
chemical component;
processing and drying methods
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Paeonia;
Plant Extracts;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(22):5839-5847
- CountryChina
- Language:Chinese
-
Abstract:
The present study evaluates different processing and drying methods and investigates their effects on the chemical components in Paeoniae Radix Alba via content determination. The fresh medicinal materials of Paeoniae Radix Alba collected from Bozhou of Anhui province were processed(boiled and peeled) and dried(hot air-dried, infrared-dried, and microwave-dried) at different temperatures(40, 50, 60 and 70 ℃), and the 11 components(monoterpene glycosides, polyphenols, tannin, and benzoic acid) in Paeoniae Radix Alba were determined by ultra-performance liquid chromatography coupled to triple quadrupole with electrospray tandem mass spectrometry(UPLC-TQ-MS). Then the compounds in processed and dried samples were analyzed by partial least squares discriminant analysis(PLS-DA) and orthogonal partial least squares discriminant analysis(OPLS-DA), and the contribution rates of differential components were evaluated by variable important in projection(VIP). The results indicated that the samples obtained by different processing and drying methods could be distinguished. Albiflorin, gallic acid, 1,2,3,4,6-pentakis-O-galloyl-β-D-glucose, and benzoic acid were the common differential components in boiled Paeoniae Radix Alba. Benzoic acid was the common differential component in peeled Paeoniae Radix Alba. Gallic acid was the common differential component in Paeoniae Radix Alba dried by different methods. The samples could not be distinguished after drying at different temperatures due to the lack of common differential components. This study is expected to provide a reference for the selection of processing and drying methods and the optimization of processing parameters.
