Isolation of Fusarium and identification of its toxins from tuberous root of Pseudostellaria heterophylla.
10.19540/j.cnki.cjcmm.20210819.101
- Author:
Qing-Song YUAN
1
;
Tao DENG
1
;
Jiu-Chun AN
1
;
Lan-Ping GUO
2
;
Shi-Hua ZHOU
1
;
Chang-Gui YANG
1
;
Bang-Xing HAN
3
;
Chuan-Zhi KANG
2
;
Xing-Peng LIU
4
;
Tao ZHOU
1
Author Information
1. Guizhou University of Traditional Chinese Medicine Guiyang 550025, China.
2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. West Anhui University Lu'an 237012, China.
4. Guizhou Institute for Food and Drug Control Guiyang 550004, China.
- Publication Type:Journal Article
- Keywords:
Fusarium;
Pseudostellaria heterophylla;
toxin chemotype;
toxins
- MeSH:
Caryophyllaceae;
Chromatography, Liquid;
Fusarium/genetics*;
Mycotoxins;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(22):5792-5796
- CountryChina
- Language:Chinese
-
Abstract:
Fusarium is the major pathogen of root rot of Pseudostellaria heterophylla. This study aims to explain the possible distribution of Fusarium species and the contamination of its toxin-chemotypes in tuberous root of P. heterophylla. A total of 89 strains of fungi were isolated from the tuberous root of P. heterophylla. Among them, 29 strains were identified as Fusarium by ITS2 sequence, accounting for 32.5%. They were identified as five species of F. avenaceum, F. tricinctum, F. fujikuroi, F. oxysporum, and F. graminearum based on β-Tubulin and EF-1α genes. LC-MS/MS detected 18, 1, and 5 strains able to produce ZEN, DON, and T2, which accounted for 62.1%, 3.4%, and 17.2%, respectively. Strain JK3-3 can produce ZEN, DON, and T2, while strains BH1-4-1, BH6-5, and BH16-2 can produce ZEN and T2. PCR detected six key synthase genes of Tri1, Tri7, Tri8, Tri13, PKS14, and PKS13 in strain JK3-3, which synthesized three toxins of ZEN, DON, and T2. Four key synthase genes of Tri8, Tri13, PKS14, and PKS13 were detected in strains BH1-4-1, BH6-5, and BH16-2, which were responsible for the synthesis of ZEN and T2. The results showed that the key genes of toxin biosynthesis were highly correlated with the toxins produced by Fusarium, and the biosynthesis of toxin was strictly controlled by the genetic information of the strain. This study provides a data basis for the targeted prevention and control of exo-genous mycotoxins in P. heterophylla and a possibility for the development of PCR for rapid detection of toxin contamination.
