Dryness comparison of different fractions of Aurantii Fructus extract on normal mice and gastrointestinal motility disorder rats and spectrum-dryness study.
10.19540/j.cnki.cjcmm.20210525.307
- Author:
Ri-Fa QIAO
1
;
Min-Yong ZHONG
1
;
Min ZHANG
2
;
Lin YANG
1
;
Xi-Qin DU
3
;
Fu-Lin TUO
3
;
Jin-Bin YUAN
1
Author Information
1. Key Laboratory of Modern Preparation of Traditional Chinese Medicine Under Ministry of Education, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
2. Key Laboratory of Modern Preparation of Traditional Chinese Medicine Under Ministry of Education, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China Nanchang Key Laboratory of Quality Control and Safety Evaluation of Traditional Chinese Medicine, Nanchang Institute for Food and Drug Control Nanchang 330004, China.
3. College of Pharmacy, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China.
- Publication Type:Journal Article
- Keywords:
Aurantii Fructus;
aquaporin;
dryness effect;
gastrointestinal motility disorder;
material basis;
spectrum-dryness relationship
- MeSH:
Animals;
Aquaporin 2;
Chromatography, High Pressure Liquid;
Citrus;
Drugs, Chinese Herbal;
Gastrointestinal Motility;
Medicine, Chinese Traditional;
Mice;
Rats
- From:
China Journal of Chinese Materia Medica
2021;46(20):5291-5303
- CountryChina
- Language:Chinese
-
Abstract:
Aurantii Fructus is a commonly used qi-regulating medicinal herb in China. Both traditional Chinese medicine theory and modern experimental research demonstrate that Aurantii Fructus has dryness effect, the material basis of which remains unclear. In recent years, spectrum-effect relationship has been widely employed in the study of active ingredients in Chinese medicinal herbs, the research ideas and methods of which have been constantly improved. Based on the idea of spectrum-effect study, the ultra-high perfor-mance liquid chromatography-quadrupole-time of flight mass spectrometry(UHPLC-Q-TOF-MS) fingerprints of different fractions of Aurantii Fructus extract were established for the identification of total components. Then, the dryness effects of the fractions on normal mice and gastrointestinal motility disorder(GMD) rats were systematically compared. Finally, principal component analysis(PCA), Pearson bivariate correlation analysis and orthogonal partial least squares analysis(OPLS) were integrated to identify the dryness components of Aurantii Fructusextract. The results showed that narirutin, naringin, naringenin, poncirin, oxypeucedanin, and eriodictyol-7-O-glucoside had significant correlations with and contributed to the expression of AQP2 in kidney, AQP3 in colon, and AQP5 in submandibular gland, which were the main dryness components in Aurantii Fructus.
