Screening of plant growth-promoting rhizobacteria and its effect on seed germination of Polygonum multiflorum.
10.19540/j.cnki.cjcmm.20210712.101
- Author:
Chang-Zheng LIU
1
;
Xiao-Lin JIANG
1
;
Qi-Zhong CAI
1
;
Liang-Yun ZHOU
1
;
Quan YANG
1
Author Information
1. Guangdong Provincial Research Center on Good Agricultural Practice & Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials, Guangzhou Comprehensive Experimental Station Chinese Materia Medica Industry Technology System, Key Laboratory for Production & Development of Cantonese Medicinal Materials Under State Administration of Traditonal Chinese Medicine, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
Polygonum multiflorum;
growth-promoting characteristics;
plant growth-promoting rhizobacteria;
strain identification
- MeSH:
Bacillus;
Fallopia multiflora;
Germination;
Seeds;
Soil Microbiology
- From:
China Journal of Chinese Materia Medica
2021;46(20):5247-5252
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the rhizobacteria and actinomycetes of Polygonum multiflorum were screened for the strains with indole acetic acid(IAA)-producing capacity by Salkowski method, the siderophore-producing strains by Chrome Azurol S(CAS) assay, and the strains with inorganic phosphorus-solubilizing capacity by PKO inorganic phosphorus medium. The strains were identified by morphological identification, physiological and biochemical characteristics, and 16 S rDNA sequences. Furthermore, the effect of growth-promoting strains on the seed germination and development of P. multiflorum was tested. The results showed that among 196 strains, two strains F17 and F42 were found to be capable of producing IAA and siderophore and solubilizing inorganic phosphorus simulta-neously. For F17 and F42, the results are listed below: 38.65 and 33.64 mg·L~(-1) for IAA production, 0.85 and 0.49 for siderophore-producing capacities(A_s/A_r), and 1.35 and 1.70 for inorganic phosphorus-solubilizing capacities(D/d), respectively. Comprehensive analysis revealed that strains F17 and F42 were identified as Pseudochrobactrum asacharolyticum and Bacillus aryabhattai, respectively, and both could significantly promote the seed germination of P. multiflorum.
