Screening of zearalenone-degrading bacteria and analysis of degradation conditions.
10.19540/j.cnki.cjcmm.20210716.101
- Author:
Tao DENG
1
;
Qing-Song YUAN
1
;
Tao ZHOU
1
;
Lan-Ping GUO
2
;
Wei-Ke JIANG
1
;
Shi-Hua ZHOU
1
;
Chang-Gui YANG
1
;
Chuan-Zhi KANG
2
Author Information
1. Guizhou University of Traditional Chinese Medicine Guiyang 550025, China.
2. National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Acinetobacter calcoaceticus;
biodegradation;
degradation;
zearalenone(ZEN)
- MeSH:
Bacteria;
Fusarium;
Mycotoxins;
Temperature;
Zearalenone
- From:
China Journal of Chinese Materia Medica
2021;46(20):5240-5246
- CountryChina
- Language:Chinese
-
Abstract:
Zearalenone(ZEN) is a mycotoxin produced by Fusarium, possessing estrogen-like effects, carcinogenicity, and multiple toxicities. To seek more efficient and practical agents for biological detoxification and broaden their application, this study isolated 194 bacterial strains from the moldy tuberous root of Pseudostellaria heterophylla, which were co-cultured with ZEN. An efficient ZEN-degrading strain H4-3-C1 was screened out by HPLC and identified as Acinetobacter calcoaceticus by morphological observation and molecular identification. The effects of culture medium, inoculation dose, culture time, pH, and temperature on the degradation of ZEN by H4-3-C1 strain were investigated. The mechanism of ZEN degradation and the degrading effect in Coicis Semen were discussed. The degradation rate of 5 μg·mL~(-1) ZEN by H4-3-C1 strain was 85.77% in the LB medium(pH 6) at 28 ℃/180 r·min~(-1) for 24 h with the inoculation dose of 1%. The degradation rate of ZEN in the supernatant of strain culture was higher than that in the intracellular fluid and thalli. The strain was inferred to secret extracellular enzymes to degrade ZEN. In addition, the H4-3-C1 strain could also degrade ZEN in Coicis Semen. If the initial content of ZEN in Coicis Semen was reduced from 90 μg·g~(-1) to 40.68 μg·g~(-1), the degradation rate could reach 54.80%. This study is expected to provide a new strain and application technology for the biological detoxification of ZEN in food processing products and Chinese medicinal materials.
