Content determination of eight phenols in Ilex hainanensis harvested at different time by HPLC-DAD.
10.19540/j.cnki.cjcmm.20210720.201
- Author:
Meng XIAO
1
;
Xiu-Wei LI
2
;
Xia WU
2
;
Wen-Wen ZHAO
2
;
Ke ZAN
3
;
Xiao-Qing CHEN
2
Author Information
1. School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China National Institutes for Food and Drug Control Beijing 102629, China.
2. School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China.
3. National Institutes for Food and Drug Control Beijing 102629, China.
- Publication Type:Journal Article
- Keywords:
HPLC-DAD;
Ilex hainanensis;
content determination;
harvest time;
hemiterpene glucosides;
phenols
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Ilex;
Phenols
- From:
China Journal of Chinese Materia Medica
2021;46(19):5038-5043
- CountryChina
- Language:Chinese
-
Abstract:
This study intends to develop a high performance liquid chromatography-diode array detection(HPLC-DAD) method for simultaneous determination of chlorogenic acid, 2-hydroxymethyl-3-hydroxyl-1-butene-4-O-β-D-(6″-O-caffeoyl)-glucopyranoside, pubescenoside B, huazhongilexone-7-O-β-D-glucopyranoside, rutin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C in Ilex hainanensis. The HPLC conditions are as follows: Waters XBridge C_(18 )column(4.6 mm×250 mm, 5 μm), mobile phase of 0.5% formic acid in water(A)-acetonitrile(B), gradient elution(0-8 min, 5%-12% B; 8-18 min, 12%-18% B; 18-30 min, 18%-25% B; 30-40 min, 25%-30% B; 40-42 min, 30%-80% B; 42-45 min, 80% B) at the flow rate of 0.8 mL·min~(-1), detection wavelengths of 282, 324, and 360 nm, column temperature of 25 ℃, and injection volume of 5 μL. The content of the 8 phenols in 8 samples was 0.30-6.29, 0.29-3.27, 0.15-10.4, 0.51-5.85, 0.49-9.02, 0.51-4.68, 1.93-13.4, and 0.87-5.95 mg·g~(-1), respectively. Moreover, the content of phenols in the samples collected in October was higher than that of samples harvested in other months. The established method is accurate and sensitive for the determination of phenols in I. hainanensis, which is useful for the quality improvement of this herbal medicine.
