Chemical constituents of steroidal saponins in rhizome of Paris polyphylla var. yunnanensis cultured in vitro.
10.19540/j.cnki.cjcmm.20210722.101
- Author:
Ying-Ying ZHANG
1
;
Lin JIN
2
;
Yu-Ying WU
3
;
Rui LI
3
;
Kai-Ling WANG
1
;
Hai-Feng LI
1
Author Information
1. College of Pharmacy, Dali University Dali 671000, China Institute of Materia Medica, Dali University Dali 671000, China Yunnan Key Laboratory of Screening and Research on Anti-pathogenic Plant Resources from Western Yunnan (Cultivation) Dali 671000, China.
2. Infectious Disease Hospital of Lijiang Lijiang 674100, China.
3. College of Pharmacy, Dali University Dali 671000, China Institute of Materia Medica, Dali University Dali 671000, China.
- Publication Type:Journal Article
- Keywords:
Paris polyphylla var.yunnanensis;
in vitro culture;
liquid chromatography-mass spectrometry;
rhizome;
steroidal saponins
- MeSH:
Chromatography, High Pressure Liquid;
Liliaceae;
Melanthiaceae;
Rhizome;
Saponins
- From:
China Journal of Chinese Materia Medica
2021;46(19):4936-4944
- CountryChina
- Language:Chinese
-
Abstract:
In this study, liquid chromatography-mass spectrometry(LC-MS) and high performance liquid chromatography(HPLC) were employed for qualitative and quantitative analysis of the steroidal saponins in rhizomes of Paris polyphylla var. yunnanensis from three different habitats cultured in vitro, in an attempt to explore whether the rhizomes of the medicinal herb cultured in vitro can synthesize the steroidal saponins, including polyphyllinsⅠ, Ⅱ, and Ⅶ, the quality markers specified in Chinese Pharmacopoeia(2020 edition). A total of 20 steroidal saponins were identified in the rhizomes from Changxin, Yunlong(S1), Fengyi, Dali(S2), and Niujie, Eryuan(S3): parisyunnanoside A and parisyunnanoside D or E, proto-polyphyllin Ⅱ, polyphyllins G and H, polyphyllinsⅠ, Ⅱ, Ⅴ, Ⅵ, and Ⅶ, dioscin, gracillin, prosapogenin A, Tg, isomer of Th, saponin Th, reclinatoside, proto-pairs D, pseudoproto-dioscin, and 23-O-glc-(23S,25R)-spirost-5-en-3β,23α,27-triol-3-O-rha-(1→2)-[ara(1→4)]-glc or 27-O-glc-(23S,25R)-spirost-5-en-3β,27α-diol-3-O-rha-(1→2)-[ara(1→4)]-glc. Among them, polyphyllinsⅠ, Ⅱ, and Ⅶ were detected in the rhizomes from S1, with the mass fraction of 0.109 1%, 0.165 2%, and 0.051 03%, respectively(total 0.325 3%). Polyphyllins Ⅱ and Ⅶ were identified in the rhizomes from S2 with the respective mass fraction of 0.192 2% and 0.074 23% and total content of 0.266 5%. Moreover, polyphyllins Ⅱ and Ⅶ were also found in the rhizomes from S3, which had the mass fraction of 0.207 7% and 0.186 9%, separately, with the total content of 0.394 6%. Thus, steroidal saponins, including the quality makers polyphyllins Ⅰ, Ⅱ, and Ⅶ recorded in Chinese Pharmacopoeia(2020 edition) can be synthesized in rhizomes of Paris polyphylla var. yunnanensis cultured in vitro, but their total content fails to meet the standard(0.60% in Chinese Pharmacopoeia). Therefore, in vitro culture of the Paris polyphylla var. yunnanensis is feasible, but the culture conditions need to be further improved.
