Expression of Runt-related Transcription Factor 3 in Human Colon Cancer Cell Line HCT-116 Resistant to 5-Fluorouracil and the Mechanism of Drug Resistance.
10.3881/j.issn.1000-503X.13598
- Author:
Shuang FAN
1
;
Xue-Liang WU
1
;
Jun XUE
1
;
Dan-Dan XU
2
;
Yan-Jun HAN
3
;
Yuan-Rui LI
4
;
Ming QU
1
Author Information
1. Department of General Surgery, the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China.
2. Central Laboratory,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China.
3. Graduate School of Hebei North University,Zhangjiakou,Hebei 075000,China.
4. College of Life Sciences,Northwest University,Xi'an 710000,China.
- Publication Type:Journal Article
- Keywords:
5-fluorouracil;
HCT-116;
human colorectal cancer;
resistant cell line;
runt-related transcription factor 3;
siRNA
- MeSH:
Cell Line, Tumor;
Colonic Neoplasms/genetics*;
Core Binding Factor Alpha 3 Subunit;
Drug Resistance, Neoplasm;
Fluorouracil/pharmacology*;
Humans;
Transcription Factor 3
- From:
Acta Academiae Medicinae Sinicae
2021;43(5):706-715
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a human colon cancer cell line HCT-116/5-FU resistant to 5-fluorouracil(5-FU)and explore the relationship between runt-related transcription factor 3(RUNX3)and drug resistance of colorectal cancer.Methods The human colon cancer cell line HCT-116/5-FU with resistance to 5-FU was established by low concentration gradient increment combined with high-dose intermittent shock.CCK-8 method was used to determine the half maximal inhibitory concentration(IC
