Role of miR-145-5p Targeting ARK5 in Regulating the Proliferation and Apoptosis of Human Epithelial Ovarian Cancer Cells.
10.3881/j.issn.1000-503X.13255
- Author:
Lei WU
1
;
Wen-Qin ZHOU
2
;
Lin-Nan YUAN
1
;
Jie LI
3
;
Mei-Li PEI
1
Author Information
1. Department of Gynecology and Obstetrics,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China.
2. Department of Gynecology and Obstetrics,Shangnan Hospital of Traditional Chinese Medicine, Shangluo,Shaanxi 726300,China.
3. Department of Pathology,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China.
- Publication Type:Journal Article
- Keywords:
ARK5;
miR-145-5p;
ovarian cancer
- MeSH:
Apoptosis/genetics*;
Carcinoma, Ovarian Epithelial/genetics*;
Cell Line, Tumor;
Cell Proliferation/genetics*;
Female;
Gene Expression Regulation, Neoplastic;
Humans;
MicroRNAs/genetics*;
Ovarian Neoplasms/genetics*
- From:
Acta Academiae Medicinae Sinicae
2021;43(5):669-676
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of miR-145-5p on the proliferation and apoptosis of human ovarian cancer cells and the possible molecular mechanisms involved.Methods Real-time quantitative PCR was performed to detect the expression of miR-145-5p in ovarian epithelial cells and ovarian cancer cells.CCK-8 and flow cytometry were used to detect the effects of miR-145-5p overexpression on the proliferation and apoptosis of ovarian cancer cells.TargetScan was employed to predict the target genes of miR-145-5p.Western blotting,dual luciferase reporter assay and rescue experiment were employed to predict and verify the underlying molecular mechanism of miR-145-5p function.Results The expression of miR-145-5p in ovarian cancer cells was significantly lower than that in normal ovarian epithelial cells(
