Application of immobilized glycosidase in the synthesis of glycoside compounds.

Jiawei Dai; Hanchi Chen; Xiao Jin; Xiaocan Mao; Linjiang Zhu; Yuele LU; Xiaolong Chen

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Application of immobilized glycosidase in the synthesis of glycoside compounds.

Author: Jiawei DAI ¹ ; Hanchi CHEN ¹ ; Xiao JIN ¹ ; Xiaocan MAO ¹ ; Linjiang ZHU ¹ ; Yuele LU ¹ ; Xiaolong CHEN ¹
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1. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, Zhejiang, China.
Publication Type:Review
Keywords: glycosidase; glycoside compounds; immobilization; non-aqueous catalysis
MeSH: Catalysis; Enzymes, Immobilized; Glycoside Hydrolases/genetics*; Glycosides/biosynthesis*; Hydrolysis
From: Chinese Journal of Biotechnology 2021;37(12):4169-4186
CountryChina
Language:Chinese
Abstract: Glycoside compounds are widely used in medicine, food, surfactant, and cosmetics. The glycosidase-catalyzed synthesis of glycoside can be operated at mild reaction conditions with low material cost. The glycosidase-catalyzed processes include reverse hydrolysis and transglycosylation, appropriately reducing the water activity in both processes may effectively improve the catalytic efficiency of glucosidase. However, glucosidase is prone to be deactivated at low water activity. Thus, glucosidase was immobilized to maintain its activity in the low water activity environment, and even in neat organic solvent system. This article summarizes the advances in glycosidase immobilization in the past 30 years, including single or comprehensive immobilization techniques, and immobilization techniques combined with genetic engineering, with the aim to provide a reference for the synthesis of glycosides using immobilized glycosidases.