Application of iPDMS protein microarray in screening of tumor-associated antigen autoantibodies.

Fan Chen; Wei Wang; Dayong GU; Yongbo Nie; Zhengqin Xiao; Kaiyu Huang; Hongwei MA; Jianan HE; Fan Yang

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Application of iPDMS protein microarray in screening of tumor-associated antigen autoantibodies.

Author: Fan CHEN ¹ ; Wei WANG ² ; Dayong GU ³ ; Yongbo NIE ³ ; Zhengqin XIAO ² ; Kaiyu HUANG ⁴ ; Hongwei MA ⁴ ; Jianan HE ⁴ ; Fan YANG ¹
Author Information

1. State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, Hubei, China.
2. Department of Laboratory Medicince, Dongguan Chang'an Hospital, Dongguan 523843, Guangdong, China.
3. Department of Laboratory Medicince, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen 518035, Guangdong, China.
4. Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, Jiangsu, China.
Publication Type:Journal Article
Keywords: VEGF121; VEGFR1; iPDMS protein microarray; tumor marker
MeSH: Adsorption; Autoantibodies; Dimethylpolysiloxanes; Protein Array Analysis; Silica Gel; Surface Properties
From: Chinese Journal of Biotechnology 2021;37(11):4075-4082
CountryChina
Language:Chinese
Abstract: The rapid screening of tumor markers is a challenging task for early diagnosis of cancer. This study aims to use highly sensitive chemiluminescent protein microarray technology to efficiently screen a variety of low abundance tumor related markers. A new material, termed integrated polydimethylsiloxane modified silica gel (iPDMS), was obtained by adding a surface polymerization initiator with olefin end to the conventional polydimethylsiloxane, and fixing into the three-dimensional structure of polydimethylsiloxane by thermal crosslinking through silicon hydrogen bonding. In order to make the iPDMS material resistant to non-specific protein adsorption, a poly(OEGMA) polymer brush was synthesized by surface-initiated atom transfer radical polymerization at the active initiation site. Finally, 20 tumor-related antigens were printed into the specific areas of the microarray by high-throughput spray printing technology, and assembled into 48-well detection microtiterplates of the iPDMS microarray. It was found the VEGFR and VEGF121 autoantibodies that obtained from 8 common tumors (breast cancer, lung cancer, colon cancer, gastric cancer, liver cancer, leukemia, lymphoma and ovarian cancer) can be used as potential tumor markers. The chemiluminescence labeled iPDMS protein microarray can be used for the screening of tumor autoantibodies at early stage.