Application of CRISPR/Cas-based biosensors for detecting nucleic acid of pathogens.

Author: Tao LIU ¹ ; Yachen TIAN ¹ ; Cheng LIU ¹ ; Shuiqin FANG ¹ ; Youxue WU ¹ ; Meijiao WU ¹ ; Bin LI ¹ ; Hao YANG ¹ ; Qing LIU ¹
Author Information

1. School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Publication Type:Review
Keywords: CRISPR/Cas; biosensor; nucleic acid detection; pathogen
MeSH: Biosensing Techniques; CRISPR-Cas Systems/genetics*; Nucleic Acids/genetics*
From: Chinese Journal of Biotechnology 2021;37(11):3890-3904
CountryChina
Language:Chinese
Abstract: Clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats -associated protein (CRISPR/Cas) has been developed as a precise, efficient, affordable and sensitive nucleic acid detection tool due to its efficient targeted binding ability and programmability. At present, biosensors based on CRISPR-Cas system have shown excellent performance in the detection of nucleic acid of pathogens, which has attracted widespread attention, and is expected to replace the conventional detection methods. This review summarizes the latest research progress of biosensors based on CRISPR/Cas system for detecting nucleic acid of pathogens.