Advances of Cas9/sgRNA delivery system for gene editing.

Author: Xuan HU ¹ ; Song WANG ¹ ; Lu YU ² ; Xiaopeng ZHANG ² ; Wei CHEN ²
Author Information

1. Science and Technology on Advanced Ceramic Fibers and Composites Laboratory, National University of Defense Technology, Changsha 410073, Hunan, China.
2. Beijing Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
Publication Type:Review
Keywords: Cas9/sgRNA delivery; gene editing; genetic therapy; nanocarrier
MeSH: CRISPR-Cas Systems/genetics*; Gene Editing; RNA, Guide/genetics*
From: Chinese Journal of Biotechnology 2021;37(11):3880-3889
CountryChina
Language:Chinese
Abstract: In the application of CRISPR genome editing, direct cellular delivery of non-replicable Cas9/sgRNA may reduce unwanted gene targeting and integrational mutagenesis, thus offering greater specificity and safety. Cas9/sgRNA delivery system holds great potential for treating genetic diseases. This review summarizes the advances of Cas9/sgRNA delivery systems and its therapeutic applications, providing new understandings and inspirations for vector design and future clinical applications.