A polycyclic aromatic hydrocarbon degrading strain and its potential of degrading phenanthrene in various enhanced systems.

Congyang Liu; Meini Wang; Jiameng Zhang; Youfen Qian; Kun Xiao; Rennü Wang; Wei Dong; Tao Pan

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A polycyclic aromatic hydrocarbon degrading strain and its potential of degrading phenanthrene in various enhanced systems.

Author: Congyang LIU ¹ ; Meini WANG ¹ ; Jiameng ZHANG ¹ ; Youfen QIAN ² ; Kun XIAO ¹ ; Rennü WANG ¹ ; Wei DONG ¹ ; Tao PAN ¹
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1. Jiangxi Province Key Laboratory of Mining and Metallurgy Environmental Pollution Control, School of Resource and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, Jiangxi, China.
2. State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou 510070, Guangdong, China.
Publication Type:Journal Article
Keywords: Moraxella; biodegradation; phenanthrene; polycyclic aromatic hydrocarbons; slurry
MeSH: Biodegradation, Environmental; Humans; Phenanthrenes; Polycyclic Aromatic Hydrocarbons; RNA, Ribosomal, 16S/genetics*; Soil Microbiology; Soil Pollutants
From: Chinese Journal of Biotechnology 2021;37(10):3696-3707
CountryChina
Language:Chinese
Abstract: Polycyclic aromatic hydrocarbons (PAHs) are a class of common environmental pollutants that pose threats to human health. In this study, a mesophilic bacterial strain CFP312 (grown at 15-37 °C, optimal at 30 °C) was isolated from PAHs-contaminated soil samples. It was identified as Moraxella sp. by morphological observation, physiological and biochemical test, and 16S rRNA gene phylogeny analysis. This is the first reported PAHs degrading strains in Moraxella. Degradation analysis showed that 84% and 90% of the loaded phenanthrene (400 mg/L) were degraded within 48 h and 60 h, and the degradation rates reached 1.21 and 1.29 mg/(L·h), respectively. During the degradation of phenanthrene, phenanthrene-3,4-dihydrodiol was detected as an intermediate. Based on this, it was proposed that double oxygenation at the positions 3 and 4 of phenanthrene was the first step of biodegradation. Adaptability of strain CFP312 to different enhanced phenanthrene-degradation systems was tested in aqueous-organic system, micellar aqueous system, and cloud point system. Strain CFP312 showed good adaptability to different systems. In addition, the bacterium can rapidly degrade the phenanthrene in contaminated soil in slurry-aqueous system, indicating great potential in environmental remediation.