Isolation and characterization of a polyurethane-degrading bacterium.

Jie HE; Anming XU; Jiawei Liu; Jie Zhou; Zhongli Cui; Weiliang Dong; Min Jiang

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Isolation and characterization of a polyurethane-degrading bacterium.

Author: Jie HE ¹ ; Anming XU ¹ ; Jiawei LIU ¹ ; Jie ZHOU ¹ ; Zhongli CUI ² ; Weiliang DONG ¹ ; Min JIANG ¹
Author Information

1. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China.
2. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
Publication Type:Journal Article
Keywords: Brevibacillus; biodegradation; plastic pollution; polyurethane
MeSH: Bacteria; Biodegradation, Environmental; Phylogeny; Polyurethanes
From: Chinese Journal of Biotechnology 2021;37(10):3675-3684
CountryChina
Language:Chinese
Abstract: Biodegradation of polyurethane (PUR) pollutants by microorganisms has received widespread attention currently. Identification of microorganisms capable of efficiently degrading PUR plastics is a key point. In this study, a strain P10 capable of degrading PUR was isolated from the plastic wastes, and identified as a bacterium belonging to the genus of Brevibacillus based on colony morphology and 16S rDNA phylogenetic analysis. Brevibacillus sp. P10 was capable of degrading 71.4% of waterborne polyurethane (Impranil DLN) after 6 days growth in MSM medium with DLN as a sole carbon source. In addition, strain P10 can use commercial PUR foam as the sole carbon source for growth. Brevibacillus sp. P10 can degrade 50 mg PUR foam after 6 days growth in MSM medium supplemented with 5% (V/V) LB after optimization of degradation conditions. This indicates that Brevibacillus sp. P10 has potential to be used in biodegradation of PUR waste.