Advances of CRISPR/dCas9 system in live cell imaging.

Author: Siyu LIN ¹ ; Xing ZHONG ² ; Lixin MA ¹ ; Jie QIAO ¹ ; Yi LIU ¹
Author Information

1. State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, Hubei, China.
2. Zhixing College, Hubei University, Wuhan 430011, Hubei, China.
Publication Type:Review
Keywords: CRISPR/dCas9; chromosome imaging; gene editing; genome imaging
MeSH: CRISPR-Cas Systems/genetics*; Chromatin; Endonucleases; Gene Editing
From: Chinese Journal of Biotechnology 2021;37(9):3061-3070
CountryChina
Language:Chinese
Abstract: The study of distinct genes, chromosomes and the spatio-temporal relationships between them is of great significance in genetics, developmental biology and biomedicine. CRISPR/Cas9 has become the most widely used gene editing tool due to its excellent targeting ability. Recently, researchers have developed a series of advanced live cell imaging techniques based on the nuclease-inactivated mutant of Cas9 (dCas9), providing rapid and convenient tools for high-resolution imaging of specific sites in the chromatin and genome. This review summarizes the advances of CRISPR/dCas9 system in live cell imaging from three aspects, including the strategies of cell delivery, optimization of the fluorescence signals, as well as orthogonal and multicolor imaging. Furthermore, we shed light on the development trends and prospects of this field.