Expression and mechanism of long non-coding RNA HCG22 in oral squamous cell carcinoma.

Yong Qiang Gao; Peng Wei Shi; Wen Kai Shi; Yi Ming Liu

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Expression and mechanism of long non-coding RNA HCG22 in oral squamous cell carcinoma.

Author: Yong Qiang GAO ¹ ; Peng Wei SHI ² ; Wen Kai SHI ³ ; Yi Ming LIU ²
Author Information

1. Dept. of Periodontics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
2. Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
3. Dept. of Hepatobiliary Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Publication Type:Journal Article
Keywords: cell invasion; cell migration; long non-coding RNA HCG22; microRNA-650; oral squamous cell carcinoma
MeSH: Carcinoma, Squamous Cell/genetics*; Cell Line, Tumor; Cell Movement; Cell Proliferation; Gene Expression Regulation, Neoplastic; Head and Neck Neoplasms; Humans; MicroRNAs/genetics*; Mouth Neoplasms/genetics*; RNA, Long Noncoding/genetics*; Squamous Cell Carcinoma of Head and Neck
From: West China Journal of Stomatology 2021;39(6):658-666
CountryChina
Language:English
Abstract: OBJECTIVES:To investigate the expression and mechanism of the long non-coding RNA (lncRNA) HCG22 in oral squamous cell carcinoma (OSCC).
METHODS:HCG22 levels were detected in the OSCC and adjacent tissues, OSCC cells, and normal oral keratinocytes. HCG22 expression in SCC-25 and HSC-3 cells was upregulated by transfection of the overexpressing plasmi dvector. Methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, and Transwell assay were employed to detect changes in cell proliferation, apoptosis, migration, and invasion ability, while Western blotting was used to detect the expression of epithelial-mesenchymal transformation-related proteins. The expression level of miR-650 in the cells was detected by real-time quantitative polymerase chain reaction (RT-qPCR), and dual-luciferase reporter gene assay was applied to assess the targeting relationship between HCG22 and miR-650.
RESULTS:Compared with that in adjacent tissues, the expression of HCG22 significantly decreased in OSCC tissues (
CONCLUSIONS:HCG22 is expressed at low levels in OSCC. Upregulation of the expression of this lncRNA can inhibit the proliferation, migration, invasion, and epithelial-mesenchymal transition of OSCC cells. The mechanism of action of HCG22 may be related to its targeted regulation of miR-650.