Effect of 17β-estradiol on the proliferation of condylar chondrocytes.
- Author:
Shuai ZHANG
1
;
Jiang Hong WANG
1
;
Li Jie TIAN
2
;
Bao Li WANG
2
;
Juan ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: 17β-estradiol; condylar chondrocyte; phosphorylated-mammalian target of rapamycin; rapamycin; temporomandibular joint osteoarthritis
- MeSH: Animals; Autophagy; Cell Proliferation; Chondrocytes; Estradiol; Estrogen Receptor alpha/metabolism*; Estrogen Receptor beta; Female; Phosphorylation; Rats
- From: West China Journal of Stomatology 2021;39(6):651-657
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To study the effects of 17β-estradiol (E2) on the regulation of the proliferation of condylar chondrocytes and provide a preliminary discussion on the role of phosphorylate-mammalian target of rapamycin (p-mTOR) in this regulatory process.
METHODS:Condylar chondrocytes were isolated from 6-week-old female rats for primary culture. Drug treatment with different concentrations of E2 and/or rapamycin (RAPA) was carried out on second-generation cells. Cell Counting Kit 8 was used to measure the cell viability of condylar chondrocytes after culture for 24, 48, or 72 h, and reverse transcription-polymerase chain reaction (RT-PCR) was applied to detect the relative gene expression of estrogen receptor alpha (ERα), estrogen receptor beta (ERβ), collagen type Ⅱ (COLⅡ), autophagy-related gene 6 (Beclin-1), and autophagy-related gene 5 (ATG-5). Western blot was employed to determine the relative protein expression of ERα, ERβ, Beclin-1, lipid-modified light chain 3B (LC3-Ⅱ), and p-mTOR.
RESULTS:E2 could significantly promote the proliferation of chondrocytes cultured
CONCLUSIONS:At a concentration of 10
