Effect of type 2 diabetes mellitus on mandibular bone regeneration and the expression of T helper cell 17/regulat-ory T cell-related factors in mice.

Ya Nan Wang; Xuan WU; Ting Ting Jia; Yao Feng; Shi Yue Liu; Xin XU; Dong Jiao Zhang

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Effect of type 2 diabetes mellitus on mandibular bone regeneration and the expression of T helper cell 17/regulat-ory T cell-related factors in mice.

Author: Ya Nan WANG ¹ ; Xuan WU ² ; Ting Ting JIA ¹ ; Yao FENG ¹ ; Shi Yue LIU ³ ; Xin XU ¹ ; Dong Jiao ZHANG ¹
Author Information

1. Dept. of Implantology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan 250012, China.
2. Dept. of Implantology, Yuehu Branch, Ningbo Dental Hospital, Ningbo 315000, China.
3. Dept. of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan 250012, China.
Publication Type:Journal Article
Keywords: T helper cell 17; bone regeneration; protein tyrosine phosphatase non-receptor type 2; regulatory T cell; type 2 diabetes mellitus
MeSH: Animals; Bone Regeneration; Diabetes Mellitus, Type 2; Forkhead Transcription Factors; Male; Mice; Mice, Inbred C57BL; TCF Transcription Factors; Th17 Cells
From: West China Journal of Stomatology 2021;39(6):642-650
CountryChina
Language:English
Abstract: OBJECTIVES:To observe the effect of type 2 diabetes mellitus (T2DM) on mandibular bone regeneration and the expression of factors related to T helper cell 17 (Th17 cell) and regulatory T cell (Treg cell) in mice.
METHODS:Thirty-six 6-week-old C57BL/6J male mice were randomly divided into normal control (NC) and T2DM groups. Fasting blood glucose levels were detected 0 d, 7 d, 14 d, and 28 d after surgery for mandibular defects. Hematoxylin-eosin (HE) staining was used in observing the bone after 7 d, 14 d, and 28 d of the healing process. Immunohistochemical staining was used in observing the expression of alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2), forkhead box protein P3 (Foxp3), retinoic acid related orphan receptor gamma T (RORγt), and protein tyrosine phosphatase non-receptor type 2 (PTPN2) after 7 d, 14 d, and 28 d of healing.
RESULTS:HE staining showed that the area with new bones in the T2DM group was significantly smaller than that in the NC group. Immunohistochemical staining showed that the expression of osteogenesis related proteins ALP and RUNX2 were significantly reduced in the T2DM group. In addition, the number of RORγt positive cells increased, whereas the number of Foxp3 positive cells and the expression PTPN2 decreased significantly in the mandibular bone defect in mice with T2DM.
CONCLUSIONS:T2DM significantly inhibit mandibular bone regeneration in mice. Decline in PTPN2 expression and the transition of Treg and Th17 may be the underlying molecular mechanisms.