Lipid Accumulation and IL-6 Production in L02 Hepatocytes Induced by Sodium Oleate: Dose and Time Dependence.
- Author:
Chen CHEN
1
;
Jun Sheng HUO
1
;
Qin ZHUO
1
;
Yan LI
1
;
Yan MA
1
;
Jing Bo WANG
1
;
Xu Dong JIA
2
Author Information
- Publication Type:Letter
- Keywords: IL-6; L02 hepatocytes; Lipid accumulation; Sodium oleate
- MeSH: Dose-Response Relationship, Drug; Hepatocytes/metabolism*; Humans; Interleukin-6/metabolism*; Lipid Metabolism; Oleic Acid/administration & dosage*; Time Factors
- From: Biomedical and Environmental Sciences 2021;34(12):998-1004
- CountryChina
- Language:English
- Abstract: To explore interleukin-6 (IL-6) production and characterize lipid accumulation in L02 hepatocytes induced by sodium oleate. L02 hepatocytes were incubated with 0, 37.5, 75, 150, 300, 600, or 1,200 μmol/L sodium oleate for 24 h, and the supernatant was collected to detect the concentration of IL-6. L02 hepatocytes were incubated with 300, 150, 75, or 0 μmol/L sodium oleate for 0-24 h. The supernatant was collected for detection of IL-6 and free fatty acids. L02 hepatocytes treated with 300 μmol/L sodium oleate for 0-24 h were stained with Oil Red O. With extended sodium oleate incubation time, IL-6 levels increased, and free fatty acids decreased. After 24 h incubation, IL-6 levels increased as sodium oleate increased from 37.5 to 300 μmol/L (
