Ionizing Radiation-Induced RPL23a Reduction Regulates Apoptosis
- Author:
Yu Xuan HE
1
;
Yi Xiao GUO
2
;
Yong ZHANG
1
;
Jun Jie HU
1
;
Wei Tao DONG
1
;
Xiang Hong DU
1
;
Xing Xu ZHAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Ionizing radiation; Spermatogonia apoptosis; Testis
- MeSH: Animals; Apoptosis/genetics*; Gene Expression Regulation; Male; Mice; Ribosomal Proteins/metabolism*; Signal Transduction; Spermatogonia/radiation effects*
- From: Biomedical and Environmental Sciences 2021;34(10):789-802
- CountryChina
- Language:English
-
Abstract:
Objective:The expression patterns of ribosomal large subunit protein 23a (RPL23a) in mouse testes and GC-1 cells were analyzed to investigate the potential relationship between RPL23a expression and spermatogonia apoptosis upon exposure to X-ray.
Methods:Male mice and GC-1 cells were irradiated with X-ray, terminal dUTP nick end-labelling (TUNEL) was performed to detect apoptotic spermatogonia
Results:Ionizing radiation (IR) increased spermatogonia apoptosis, the expression of RPL11, MDM2 and p53, and decreased RPL23a expression in mice spermatogonia
Conclusion:These results suggested that IR reduced RPL23a expression, leading to weakened the RPL23a-RPL11 interactions, which may have activated p53, resulting in spermatogonia apoptosis. These results provide insights into environmental and clinical risks of radiotherapy following exposure to IR in male fertility. The graphical abstract was available in the web of www.besjournal.com.
