2-Hexyl-4-Pentylenic Acid (HPTA) Stimulates the Radiotherapy-induced Abscopal Effect on Distal Tumor through Polarization of Tumor-associated Macrophages.
- Author:
Wen Hua DUAN
1
;
Li Ya JIN
1
;
Zu Chao CAI
1
;
David LIM
2
;
Zhi Hui FENG
1
Author Information
- Publication Type:Journal Article
- Keywords: 2-hexyl-4-pentylenic acid (HPTA); Abscopal effect; Angiogenesis; Breast cancer; Polarization; Radiotherapy (RT); Tumor-associated macrophages (TAMs)
- MeSH: Animals; Antineoplastic Agents/therapeutic use*; Cell Proliferation/radiation effects*; Combined Modality Therapy; Cytokines/immunology*; Fatty Acids, Unsaturated/therapeutic use*; Female; Mammary Neoplasms, Experimental/radiotherapy*; Rats; Tumor-Associated Macrophages/radiation effects*
- From: Biomedical and Environmental Sciences 2021;34(9):693-704
- CountryChina
- Language:English
-
Abstract:
Objective:The aim of this study was to explore the effects of 2-hexyl-4-pentylenic acid (HPTA) in combination with radiotherapy (RT) on distant unirradiated breast tumors.
Methods:Using a rat model of chemical carcinogen (7,12-dimethylbenz[a]anthracene,DMBA)-induced breast cancer, tumor volume was monitored and treatment response was evaluated by performing HE staining, immunohistochemistry, immunofluorescence, qRT-PCR, and western blot analyses.
Results:The results demonstrated that HPTA in combination with RT significantly delayed the growth of distant, unirradiated breast tumors. The mechanism of action included tumor-associated macrophage (TAM) infiltration into distant tumor tissues, M1 polarization, and inhibition of tumor angiogenesis by IFN-γ.
Conclusion:The results suggest that the combination of HPTA with RT has an abscopal effect on distant tumors
