Analysis of the Electrophoretic Profiles of Prion Protein in Carcinous and Pericarcinous Lysates of Six Different Types of Cancers.

Wei Wei; Yue Zhang WU; Kang Xiao; Guo Hui XU; Yun Tao Song; Qi Shi; Xiao Ping Dong

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Analysis of the Electrophoretic Profiles of Prion Protein in Carcinous and Pericarcinous Lysates of Six Different Types of Cancers.

Author: Wei WEI ¹ ; Yue Zhang WU ² ; Kang XIAO ² ; Guo Hui XU ¹ ; Yun Tao SONG ¹ ; Qi SHI ² ; Xiao Ping DONG ^{3
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Author Information

1. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Head and Neck Surgery Department, Peking University Cancer Hospital & Institute, Beijing 100142, China.
2. State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University), National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
3. State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases (Zhejiang University), National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
4. Center for Global Public Health, Chinese Center for Disease Control and Prevention, Beijing 102206, China
5. Wuhan Institute of Virology, Chinese Academy of Science, Wuhan 430071, Hubei China
6. China Academy of Chinese Medical Sciences, Beijing 100700, China.
Publication Type:Journal Article
Keywords: Cancer; Electrophoretic profiles; Prion protein
MeSH: Animals; Blotting, Western; Brain; Brain Chemistry; Cricetinae; Electrophoresis, Polyacrylamide Gel; Humans; Neoplasms/chemistry*; Prion Proteins/analysis*
From: Biomedical and Environmental Sciences 2021;34(9):683-692
CountryChina
Language:English
Abstract: Objective:To find the different electrophoretic profiles of prion protein in carcinous and individual pericarcinous tissues in lysates of gastric, colon, liver, lung, thyroid, and laryngeal cancers.
Methods:Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot were used to test the amounts and electrophoretic patterns of total PrP and the tolerance of PK (protease K) digestion among six various cancer tissue types.
Results:A mass of PrP signals with a large molecular weight were identified in the homogenates of peripheral tissues. The amounts and electrophoretic patterns of total PrP did not differ significantly between carcinous and pericarcinous tissues. PrPs in all types of the tested cancer samples were PK sensitive but showed diversity in the tolerance of PK digestion among various tissue types.
Conclusions:The study revealed that the included electrophoretic patterns of carcinous and pericarcinous tissues were almost similar. Unlike PrP-specific immunohistochemical assay, evaluation of PrP electrophoretic patterns in the peripheral organs and tissues by Western blot does not reflect tumor malignancy.