TRPV4 channel mediates the increase of pulmonary microvascular endothelial permeability in rats with chronic hypoxic pulmonary hypertension.
- Author:
Hai-Xia JIAO
1
;
Sheng-Xia YUAN
2
;
Yan-Zhen HUANG
2
;
Qiao-Wen SU
2
;
Rui-Lan HE
1
;
Zhi-Juan WU
1
;
Mo-Jun LIN
1
Author Information
1. School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, China.
2. The Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases, Fujian Medical University, Fuzhou 350122, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Endothelial Cells;
Hypertension, Pulmonary;
Hypoxia/complications*;
Lung;
Permeability;
Rats;
TRPV Cation Channels/genetics*
- From:
Acta Physiologica Sinica
2021;73(6):867-877
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of the present study was to investigate the effect of transient receptor potential vanilloid 4 (TRPV4) channel on the permeability of pulmonary microvascular endothelial cells (PMVECs) in rats with chronic hypoxia-induced pulmonary hypertension (CHPH), so as to clarify the mechanism of vascular endothelial dysfunction during the occurrence of pulmonary hypertension (PH). CHPH rat model was established by exposure to chronic hypoxia (CH) for 21 days. Primary PMVECs were cultured by adherent tissue blocks at the edge of the lung. The permeability coefficient of primary cultured PMVECs was detected by fluorescein isothiocyanate (FITC)-dextran. The structure of tight junction (TJ) was observed by transmission electron microscope. The expression of TRPV4 and TJ-related proteins, such as, Occludin, Claudin-5, ZO-1 were examined by real-time fluorescence quantitative PCR and Western blotting. The intracellular calcium concentration ([Ca