Effect of moxibustion at "Zusanli" (ST 36) and "Shenshu" (BL 23) on intestinal flora in adjuvant arthritis rats.
10.13703/j.0255-2930.20201010-k0003
- Author:
Yan ZHU
1
;
Min ZHANG
2
;
Chen ZHAO
2
Author Information
1. First Department of Geriatrics, Second Affiliated Hospital of Anhui University of CM, Hefei 230061, China.
2. Graduate School of Anhui University of CM.
- Publication Type:Journal Article
- Keywords:
adjuvant arthritis;
inflammatory factors;
intestinal flora;
moxibustion;
rheumatoid arthritis
- MeSH:
Animals;
Arthritis, Experimental/therapy*;
Arthritis, Rheumatoid;
Gastrointestinal Microbiome;
Moxibustion;
Rats;
Rats, Wistar
- From:
Chinese Acupuncture & Moxibustion
2021;41(10):1119-1125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of moxibustion at "Zusanli" (ST 36) and "Shenshu" (BL 23) on inflammatory factors and intestinal flora in the rats with adjuvant arthritis.
METHODS:A total of 36 Wistar rats were randomized into a normal group, a model group and a moxibustion group, 12 rats in each one. In the model group and the moxibustion group, the adjuvant arthritis model was established by a compound method, including the environmental factors, i.e. wind, cold and damp, and Freund's complete adjuvant. In the moxibustion group, moxibustion intervention was exerted at "Zusanli" (ST 36) and "Shenshu" (BL 23), for 20 min at each acupoint, once daily, consecutively for 21 days. The paw swelling degree and arthritis index (AI) score were observed before and after intervention in the rats of each group. Using real-time fluorescence quantitative PCR method (real-time PCR) and Western blot method, the mRNA and protein expressions of inflammatory factors of colon tissue, i.e. interleukin (IL) 1β, tumor necrosis factor-α (TNF-α), IL-6, were detected after intervention in the rats of each group. The intestinal flora was detected with 16SrRNA sequencing technology after intervention in the rats of each group.
RESULTS:Compared with the normal group, the paw swelling degree and AI score were increased in the rats of the model group (
CONCLUSION:Moxibustion at "Zusanli" (ST 36) and "Shenshu" (BL 23) relieves the joint symptoms of adjuvant arthritis rats and inhibits the expressions of inflammatory factors, which is probably related to the regulation of the structure of intestinal flora.
