Effect of electroacupuncture on expression of IL-23/IL-17 axis and TLR4 in infarcted tissue in rats with myocardial infarction.
10.13703/j.0255-2930.20200824-k0001
- Author:
Ke SUN
1
;
Jia-Hong WU
1
;
Hua BAI
1
;
Sen-Lei XU
1
;
Xue-Feng XIA
1
;
Yu-Chen LIU
1
;
Sheng-Feng LU
2
,
3
;
Yi-Huang GU
1
;
Hong-Ru ZHANG
1
Author Information
1. College of Acupuncture-Moxibustion and Tuina, College of Health and Rehabilitation, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China.
2. College of Acupuncture-Moxibustion and Tuina, College of Health and Rehabilitation, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China
3. Key Laboratory of Acupuncture and Medicine of Ministry of Education, Nanjing University of Chinese Medicine.
- Publication Type:Journal Article
- Keywords:
IL-23/IL-17 axis;
Toll-like receptor 4;
electroacupuncture;
myocardial infarction
- MeSH:
Animals;
Electroacupuncture;
Interleukin-17/genetics*;
Interleukin-23/genetics*;
Male;
Myocardial Infarction/therapy*;
Rats;
Rats, Sprague-Dawley;
Toll-Like Receptor 4/genetics*
- From:
Chinese Acupuncture & Moxibustion
2021;41(9):1023-1028
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To observe the effect of electroacupuncture (EA) on expression of interleukin (IL) -23/IL-17 axis and Toll-like receptor 4 (TLR4) in the infarcted tissue in rats with myocardial infarction (MI), and to explore the mechanism of EA on alleviating MI injury.
METHODS:Forty male SD rats were randomly divided into a sham-operation group, a sham-operation plus EA group, a model group and an EA group, 10 rats in each group. The MI models were established by ligation of left anterior descending coronary artery in the model group and EA group, while only threading was performed in the sham-operation group and sham-operation plus EA group. The rats in the sham-operation plus EA group and EA group were treated with EA at "Neiguan" (PC 6), disperse-dense wave, 2 Hz/100 Hz, 2 mA, once a day, 20 min each time, for 3 days. After the intervention, the ejection fraction (EF) was measured by echocardiography to evaluate the cardiac function; the infarct area was measured by TTC staining; the HE staining was used to observe the morphological changes of myocardial tissue; the levels of IL-23 and IL-17 in infarcted tissue were detected by ELISA; the protein expression of TLR4 in infarcted tissue was detected by Western blot.
RESULTS:Compared with the sham-operation group, the EF was decreased (
CONCLUSION:EA may alleviate the excessive inflammatory response after MI by inhibiting the expression of IL-23/IL-17 axis in MI rats, and TLR4 may be involved during the process.
