Determination of five prostaglandin analogs in eyelash enhancing cosmetics by LC-MS/MS
10.19428/j.cnki.sjpm.2022.20643
- VernacularTitle:化妆品睫毛增长液中前列腺素类似物的高效液相色谱-串联质谱法测定
- Author:
Yong XU
1
;
Chen PAN
1
;
Jing HAN
1
;
Xingsheng PENG
1
;
Rong ZHENG
1
Author Information
1. NMPA Key Laboratory for Monitoring and Evaluation of Cosmetics， Shanghai Institute for Food and Drug Control， Shanghai 201203， China
- Publication Type:Journal Article
- Keywords:
eyelash enhancing;
bimatoprost;
tafluprost ethyl amide;
latanoprost;
travoprost;
tafluprost;
LC-MS/MS
- From:
Shanghai Journal of Preventive Medicine
2022;34(2):177-182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine bimatoprost， tafluprost ethyl amide， latanoprost， travoprost and tafluprost in eyelash enhancing cosmetics by establishing a LC-MS/MS method. Methods The samples were extracted with a 50% acetonitrile water solution. A salt mixture（4 g NaCl， 1 g MgSO4） was added to the solution to induce phase separation. After centrifugation and filtration， the analysis of five prostaglandin analogs was performed with an Agilent Poroshell 120 PFP-C18 （2.7 μm， 2.1 mm×100 mm） column， using 0.02% formic acid containing 5 mmol·L-1 Acetic acid amine and acetonitrile by gradient elution at a flow rate of 0.5 mL·min-1. The analytes were detected with electrospray ionization source in positive ion mode （ESI+） and multiple reaction monitoring （MRM）， and quantified by external standard curve. Results The results showed that it had a good linearity in the range of locatable ambit of concentration with correlation coefficients （r） larger than 0.999. The detection limit of five prostaglandin analogs （LOD） was 0.000 2‒1.5 μg·g-1. The spiked recoveries were 93.2% to 103.5% with a relative standard deviation （RSD） of 1.2% to 3.4%. Conclusion The method is simple， rapid and highly sensitive. It is suitable for the determination of five prostaglandin analogs in eyelash enhancing cosmetics.
