Performance of loop-mediated isothermal amplification (LAMP) assay for detection of Schistosoma japonicum infection in Oncomelania snails in schistosomiasis transmission-interrupted regions
10.16250/j.32.1374.2021065
- VernacularTitle:血吸虫病传播阻断地区环介导等温扩增技术 检测钉螺血吸虫感染性效果
- Author:
Feng CHEN
1
;
Ke-rong LI
1
;
Wen-bao LI
2
;
Shu-hui TIAN
2
;
Ping LI
2
;
Yin-jiao ZHAO
1
;
Jing YANG
1
;
Hua YANG
1
;
Bing-rong LUO
1
;
Jun-hua MA
1
;
Ming-ming HAO
1
;
Shao-rong CHEN
1
;
Yu-hua LIU
1
;
Tian-peng LUO
1
Author Information
1. Dali Bai Autonomous Prefecture Institute of Schistosomiasis Control, Dali, Yunnan 671000, China
2. Heqing County Institute of Schistosomiasis Control, Yunnan Province, China
- Publication Type:Journal Article
- Keywords:
Oncomelania snail;
Loop-mediated isothermal amplification;
Transmission interruption;
Yunnan Province
- From:
Chinese Journal of Schistosomiasis Control
2022;34(1):81-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effectiveness of loop-mediated isothermal amplification (LAMP) assay and microscopic examinations for detection of Schistosoma japonicum infections in Oncomelania hupensis in transmission-interrupted regions, so as to provide insights into the optimization of snail surveillance tools in these regions. Methods Four hilly schistosomiasis-endemic villages where transmission interruption was achieved were selected in Heqing County of Yunnan Province as the study villages, including Xinzhuang and Gule villages in hilly regions and Lianyi and Yitou villages in dam regions. Snail survey was performed by means of systematic sampling combined with environmental sampling in July 2018. All captured snails were identified for S. japonicum infections using microscopy. In addition, 10 to 20 snails were randomly sampled from each snail habitat following microscopy, numbered according to environments and subjected to LAMP assay. The positive rate of settings with S. japonicum-infected snails was compared among villages. Results A total of 7 949 living snails were captured from 83 snail habitats in 4 villages, and no S. japonicum infection was detected in snails. There were 226 mixed samples containing 1 786 snails subjected to LAMP assay, and positive LAMP assay was found in 3 mixed samples from 3 snail habitats in 2 dam villages. The positive rates of settings with S. japonicum-infected snails were comparable between Lianyi Village (one setting) and Yitou Village (2 set tings) (5.89% vs. 14.29%, P = 0.344). However, the overall positive rate of settings with S. japonicum-infected snails was significantly higher in dam villages (9.67%, 3/31) than in hilly villages (0) (P = 0.048). Conclusions LAMP assay is more sensitive to detect S. japonicum infections in O. hupensis than conventional microcopy method, which may serve as a supplementary method for detection of S. japonicum infections in O. hupensis in high-risk snail habitats in hilly transmission-interrupted regions.
