Study on chemical constituents and in vitro antioxidant activity of ethanol extract of Sabia parviflora
- VernacularTitle:小花清风藤醇提物的化学成分及体外抗氧化活性研究
- Author:
Yongqiang ZHOU
1
;
Chunli ZHAO
1
;
Xin YIN
1
;
Tao ZHOU
1
;
Wei HAN
1
;
Yongping ZHANG
1
Author Information
1. College of Pharmacy，Guizhou University of Traditional Chi nese M edicine，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
Sabia parviflora;
chemical constituent;
70％ ethanol extract;
antioxidant activity
- From:
China Pharmacy
2022;33(5):530-534
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To separate and identif y the chemical constituen ts in 70％ ethanol extract of Sabia parviflora ，and to preliminarily evaluate their in vitro antioxidant activity. METHODS The chemical constituents were separated and purified by silica gel，ODS reversed-phase silica gel ，Sephadex-LH20 column and preparative high performance liquid chromatography. The structures of the isolated compounds were identified by 1H-NMR，13C-NMR and ESI-MS. The in vitro antioxidant activities of the compounds were investigated by 2，2-diphenyl-1-picrylhydrazyl radical （DPPH·），2，2′-azino-bis（3-ethylbenzothiazoline-6- sulfonate）diammonium radical （ABST+）and hydroxyl radical （OH·）. RESULTS A total of 9 compounds were isolated from the 70％ ethanol extracts of S. parviflora . They were identified as rutin （1），diiononyl phthalate （2），dibutyl phthalate （3），vomifoliol （4），rhododendrol（5），quercetin-3-O-gentiobioside（6），narcissoside（7），kaempferol-3-O-rutinoside（8）and bonaroside （9）. The in vitro antioxidant results showed that compound 1-9 showed certain in vitro antioxidant activity ，and the half scavenging concentrations of compound 1，6，7 and 8 to DPPH ·，ABST+，OH·were lower than 70 μg/mL. CONCLUSIONS Vomifoliol， rhododendrol and bonaroside are isolated from S. parviflora for the first time ，and rutin ，quercetin-3-O-gentiobioside，narcissoside and kaempferol- 3-O-rutinoside show good in vitro antioxidant activity.
