Experimental Study on Separation of Fetal Nucleated Red Blood Cell from the Whole Blood Using Microfluidic Chip
10.16156/j.1004-7220.2021.06.11
- VernacularTitle:有核红细胞全血分选微管芯片实验研究
- Author:
Jie LIU
1
;
Jiarong ZHANG
2
;
Yan ZHUANG
3
;
Yinyan HE
3
;
Shenghong ZHANG
1
;
Xin CHEN
1
;
Xiaobo GONG
1
Author Information
1. Department of Engineering Mechanics, Shanghai Jiao Tong University
2. Department of Gynaecology and Obstetrics, Zhongshan Hospital, Fudan University
3. Department of Gynaecology and Obstetrics, Shanghai General Hospital
- Publication Type:Journal Article
- Keywords:
noninvasive prenatal diagnosis;
fetal nucleated red blood cells（fNRBCs）;
rolling adhesion;
microfluidic chip
- From:
Journal of Medical Biomechanics
2021;36(6):E903-E909
- CountryChina
- Language:Chinese
-
Abstract:
Objective To seperate fetal nucleated red blood cells (fNRBCs) from the whole maternal peripheral blood effectively by designing a circular channel microfluidic chip. Methods A microfluidic chip is designed by utilizing the margination in blood flow and the specific adhesion characteristics of immuno-agent anti-CD147. With the whole umbilical cord blood, the effects of different shear forces on the enrichment of fNRBCs was studied by immunofluorescence counting. Results Increasing shear rate in microfluidic chip could improve the number of captured fNRBCs compared with the static adhesion. With the increase of shear rate of blood flow, the number of the captured cells increased at first, and then decreased. Conclusions The use of microfluid chip can effectively seperate fNRBCs from the whole blood. The results provide an experimental reference for the non-invasive prenatal diagnosis research and the exploration on the mechanism of fetal cell migration.
