Cyclic Stretch Promotes Migration of MC3T3-E1 Cells via Increasing Runx2 Expression
10.16156/j.1004-7220.2022.01.25
- VernacularTitle:周期性机械牵拉通过上调Runx2表达促进MC3T3-E1细胞迁移
- Author:
Xu YAN
1
;
Zitong LI
2
;
Jiting LIU
2
;
Yingxin QI
2
;
Jiong MEI
1
Author Information
1. Department of Orthopedics，Shanghai Sixth People’s Hospital
2. Institute of Mechanobiology and Medical Engineering，School of Life Science and Biotechnology，Shanghai Jiao Tong University
- Publication Type:Journal Article
- Keywords:
cyclic mechanical stretch;
cell migration;
osteoblast;
fracture healing
- From:
Journal of Medical Biomechanics
2022;37(1):E162-E168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of cyclic stretch on migration of MC3T3-E1 cells and its related mechanism. Methods The strain loading system was used to stretch MC3T3-E1 cells cultured in vitro with 15% amplitude, to simulate the mechanical condition in vivo. The wound healing assay was used to detect the migration of MC3T3-E1 cells. Western blotting was used to test Runx2 expression. RNA interfering was used to decrease Runx2 expression. Results Cyclic mechanical stretch with 15% amplitude, 1.25 Hz frequency and lasting for 24 hours could promote the migration of MC3T3-E1 cells and increase the expression level of Runx2. Runx2 interference inhibited the migration of MC3T3-E1 cells in static culture condition. Interference with Runx2 expression in MC3T3-E1 cells could partially reduce the positive effect of cyclic mechanical stretch on cell migration. Conclusions Cyclic stretch can promote the migration of MC3T3-E1 cells, and Runx2 may play an important role in this process. This study provides experimental basis for finding innovative clinical treatment method to promote fracture healing.
