Research progress of proximity labeling technology based on biotin ligase in proteomics
10.11665/j.issn.1000-5048.20220103
- VernacularTitle:基于生物素连接酶的邻近标记技术在蛋白质组学中的研究进展
- Author:
Yuxin ZHANG
1
;
Ming DING
;
Jun LIU
Author Information
1. 中国药科大学新药筛选中心
- Publication Type:Journal Article
- Keywords:
adjacent protein markers;
biotinylation;
protein-protein interaction;
BioID;
BioID2;
TurboID;
advances
- From:
Journal of China Pharmaceutical University
2022;53(1):18-24
- CountryChina
- Language:Chinese
-
Abstract:
Proximity-dependent biotinylation (PDB) uses biotin ligase fused to the protein of interest to biotinylate adjacent proteins, purify them with streptavidin beads, and then identify the biotinylated protein by mass spectrometry.This technology can be used to detect transient and/or low affinity interactions, provide a chance to learn more about membrane-less organelles and other subcellular structures that cannot be easily isolated or purified, and fill the gap in traditional methods.This article summarizes the technological development and application of PDB in recent years.
