Effects of a red light-emitting diode on the proliferation and osteogenic differentiation of stem cells from the apical papilla
10.12016/j.issn.2096-1456.2022.05.003
- Author:
SU Yutong
1
;
HOU Lan
1
;
JIANG Bing
1
;
ZHENG Genzi
1
;
LIU Yuan
1
;
WANG Yao
1
Author Information
1. The Affiliated Stomatology Hospital of Southwest Medical University
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
human stem cells from apical papilla;
photobiomodulation;
red LED;
cell proliferation;
alkaline phosphatase;
osteocalcin;
osteopontin;
osteogenic differentiation;
tissue engineering;
stem cell-based therapy
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2022;30(5):321-329
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of red LEDs on the proliferation and osteogenic differentiation of human stem cells from apical papilla (hSCAPs).
Methods:hSCAPs were obtained by isolation, culture and flow cytometry in vitro and irradiated with 1, 3, 5, and 7 J/cm2 red LEDs. The proliferation of hSCAPs was detected using a CCK-8 assay. The osteogenic differentiation of hSCAPs was evaluated using alkaline phosphatase (ALP) staining, ALP activity assay and Alizarin red quantitative detection. The effect of 5 J/cm2 red LEDs on the expression levels of the ALP, Runx2, OCN, OPN and BSP genes and proteins was detected by RT-PCR and western blot, respectively.
Results: Red LEDs at 1, 3, 5, and 7 J/cm2 promoted the proliferation of hSCAPs (P < 0.05). The effects of red LEDs with different light energies on the proliferation of hSCAPs were different at different time points (P < 0.05). On the 7th and 14th days after irradiation, red LEDs promoted the osteogenic differentiation of hSCAPs, and the effect of 5 J/cm2 red LEDs was the most obvious under osteogenic induction culture conditions (P<0.05). Red LEDs (5 J/cm2) promoted the expression of the ALP, Runx2, OCN, OPN and BSP genes and proteins (P < 0.05).
Conclusion :Red LEDs promoted the proliferation and osteogenic differentiation of hSCAPs.
- Full text:LED红光对人根尖乳头干细胞增殖和成骨分化的影响.pdf
