D-methionine eradicates Porphyromonas gingivalis biofilms by suppressing the cyclic di-GMP level
10.12016/j.issn.2096-1456.2022.05.002
- Author:
XIE Lili
1
;
ZHANG Huiyan
2
;
WANG Zixuan
2
;
LI Birong
2
;
LI Zhen
2
;
MENG Weiyan
3
Author Information
1. Department of Oral Geriatrics, Hospital of Stomatology, Jilin University
2. 1.Department of Dental Implantology, Hospital of Stomatology, Jilin University 2.Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling
3. Department of Dental Implantology, Hospital of Stomatology
- Publication Type:Journal Article
- Keywords:
biofilm;
Porphyromonas gingivalis;
D-Methionine;
cyclic diguanosine monophosphate;
periodontitis;
peri-implantitis;
exopolysaccharide;
cell membrane;
high performance liquid chromatography
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2022;30(5):314-320
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanisms by which D-methionine (D-Met) eradicates Porphyromonas gingivalis biofilms by suppressing cyclic dimeric GMP (c-di-GMP) levels.
Methods :Cell viability, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured to determine the effective concentrations of D-Met, which were subsequently used in the following experiments. During the P. gingivalis biofilm formation inhibition experiment and the mature biofilm disassembly experiment, biofilm biomass, exopolysaccharide (EPS), biofilm morphology, integrity of the cell membrane, and the level of c-di-GMP were determined.
Results : D-Met < 40 mmol/L was biocompatible. During the biofilm formation inhibition and mature biofilm disassembly experiments, D-Met ≥ 20 mmol/L decreased the biofilm biomass and the production of EPS. SEM analysis showed that the extracellular matrix and bacterial density were drastically reduced by D-Met ≥ 20 mmol/L. TEM detection showed that 35 mmol/L D-Met ruptured the cell membrane during biofilm formation and increased the permeability of the cell membrane in the disassembly phase of mature biofilms. C-di-GMP levels decreased with increasing concentrations of D-Met in a concentration-dependent manner.
Conclusion : D-Met ≥ 20 mmol/L could eradicate P. gingivalis biofilms by suppressing c-di-GMP levels.
- Full text:D-甲硫氨酸通过降低环二鸟苷酸清除牙龈卟啉单胞菌生物膜.pdf
