GC-MS analysis and anti-inflammatory activity of low polarity parts from 3 species of Sabia genus
- VernacularTitle:3种清风藤属植物低极性部位GC-MS分析及抗炎活性
- Author:
Renjie CUI
1
,
2
;
Dan HU
3
;
Lulu DENG
2
,
3
;
Jiang LI
2
,
3
;
Shuzhen MU
2
Author Information
1. College of Pharmacy，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
2. Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences，Guiyang 550014，China
3. State Key Laboratory of Functions and Ap plications of Medicinal Plants，Guizhou Medical University，Guiyang 550014，China
- Publication Type:Journal Article
- Keywords:
Sabia genus;
Sabia campanulata;
Sabia
- From:
China Pharmacy
2022;33(4):446-451
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the component characteristics and anti-infla mmatory activities of the low polarity parts from Sabia campanulata ，S. discolor and S. parviflora . METHODS Gas chromatography-mass spectrometry was used to analyze the components of low polarity parts eluted by petroleum ether from petroleum ether extract of dried stems and leaves of S. campanulata，S. discolor and S. parviflora . The chromatographic peaks were retrieved by both NIST 17 and Wiley 275 database， and their structures of each compound were compared and identified manually. The relative percentage content of each component was calculated by peak area normalization method. With indomethacin as positive control and the inhibition rate of nitric oxide （NO）production as the index ，the effects of the low polarity parts of the above three species of Sabia genus on the production of NO in lipopolysaccharide-induced human rheumatoid arthritis fibroblast-like synoviocytes （RA-FLS） and mouse mononuclear macrophage RAW 264.7 were compared ；the half inhibitory concentration （IC50）was calculated. RESULTS Totally 70，40 and 33 chemical components were identified respectively from the low polarity parts of S. campanulata ，S. discolor and S. parviflora ， including long-chain fatty hydrocarbons ，long-chain alcohol/aldehyde/ester ，monoterpenes；but there were only 5 common components in the three species ，such as dodecane and docosane，etc. S. campanulata contained abundant terpenoids （40.89％）； the relative percentage content of triterpenoids was high in S. discolor （30.29％）；the relative percentage content of long-chain fatty hydrocarbons was high in S. parviflora （87.70％）. The values of IC 50 of the low polarity parts of the above three species of Sabia genus to the production of NO in RA-FLS cells were 2.22，1.69 and 4.15 μg/mL，respectively. The values of IC 50 of them to the production of NO in RAW 264.7 cells were 1.23，4.02 and 3.05 μg/mL respectively，and all of them were lower than indomethacin （12.97，10.66 μg/mL）. CONCLUSIONS The components of the low polarity parts fr om the above three species of Sabia genus are mainly long-chain fatty hydrocarbons ， monoterpenes and long-chain alcohol/aldehyde/ester ， and all of them have certain anti-inflammatory activities.
