Pharmacokinetic difference of Shenxiong glucose injection in normal and acute myocardial ischemia rats
- VernacularTitle:参芎葡萄糖注射液在正常和急性心肌缺血大鼠体内的药动学差异
- Author:
Jingya ZHANG
1
,
2
;
Liqin LIU
1
,
2
;
Rong LI
1
,
2
;
Yuan LU
1
;
Jie PAN
3
;
Ting LIU
1
;
Jia SUN
1
Author Information
1. Guizhou Key Laboratory of Pharmaceutics/State Key Laboratory of Efficacy and Utilization of Medicinal Plants，Guizhou Medical University，Guiyang 550004，China
2. School of Pharmacy，Guizhou Medical University，Guiyang 550004，China
3. Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine of Ministry of Educatio n，Guizhou Medical University，Guiyang 550004，China
- Publication Type:Journal Article
- Keywords:
Shenxiong glucose injection;
acute myocardial
- From:
China Pharmacy
2022;33(4):433-438
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To compare the phar macokinetics o f ligustrazine hydrochlori de，salvianic acid and rosemarinic acid from Shenxiong glucose injection （SGI）in normal and acute myocardial ischemia （AMI）rats. METHODS Male SD rats were randomly divided into normal group and model group ，with 9 rats in each group. AMI model was established by isoproterenol hydrochloride modeling method. Three rats in each group were selected for model verification. The remaining 6 rats in each group were given SGI （1.2 mL/kg）or equal volum of normal saline via tail vein ；0.3 mL blood was collected through orbital venous bush 0.083，0.167，0.333，0.5，0.75，1，1.5，2，3，5 h after administration. Using luteoloside as internal standard ，the plasma concentrations of ligustrazine hydrochloride ，salvianic acid and rosemarinic acid were determined by ultra performance liquid chromatography-tandem mass spectrometry. Pharmacokinetic parameters were fitted by WinNonlin 8.1 software，and statistical analysis was performed by SPSS 18.0 software. RESULTS The linear ranges of ligustrazine hydrochloride ，salvianic acid and rosmarinic acid were 0.06-29.96，0.01-5.15 and 0.006-3.09 μ g/mL（all r＞0.99），respectively. The results of methodological investigation were all in line with the requirements of Chinese Pharmacopoeia （2020 edition）. Compared with normal rats ，CLz of ligustrazine hydrochloride in AMI model rats was significantly increased （P＜0.05）；t1/2 and Vz of salvianic acid were significantly prolonged or increased （P＜0.05）；but the cmax and AUC 0－5 h were significantly decreased （P＜0.05）；AUC0－5 h of rosmarinic acid was significantly decreased （P＜0.05）. CONCLUSIONS The exposure levels of salvianic acid and rosmarinic acid in SGI are lower in AMI model rats than in normal rats ，and the elimination of ligustrazine hydrochloride in AMI model rats is stronger than that in normal rats.
