Toluene diisocyanate induces liver injury and affects HO-1 expression in rats
10.3969/j.issn.1006-2483.2022.01.005
- VernacularTitle:甲苯二异氰酸酯诱导大鼠肝损伤及对HO-1表达的影响
- Author:
Baofeng LIU
1
;
Xudong LI
1
;
Ning HE
1
;
Runan QIN
1
;
Huijing TANG
1
;
Qiang ZENG
1
Author Information
1. Tianjin Centers for Disease Control and Prevention , Tianjin 300011 , China
- Publication Type:Journal Article
- Keywords:
Toluylene diisocyanate;
Liver;
Oxidative stress;
Heme oxygenase-1
- From:
Journal of Public Health and Preventive Medicine
2022;33(1):22-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of subchronic inhalation of toluene diisocyanate (TDI) on the pathological changes, oxidative stress damage, and HO-1 expression levels in rat liver tissues. Methods Forty healthy 3-week-old SPF-grade Sprague-Dawley male rats were randomly divided into 4 groups (control group, low-dose group, medium-dose group, and high-dose group), each with 10 rats. The rats were placed in a HOPE-MED 8050A movable poison cabinet in a cage. TDI was administered to animals by inhalation at doses of 0, 3.06 mg/m3, 12.25 mg/m3, and 49.00 mg/m3, respectively, for 6 hours a day and 5 days a week, and continuously for 13 weeks. The control group was exposed to fresh air. The effect of TDI on pathological changes, oxidative stress damage and HO-1 expression in rat liver tissues was examined. Results Compared with the control group, the rats in the medium and high-dose TDI-exposed groups exhibited vacuolar changes, hepatocyte swelling, steatosis and other pathological changes. With the increase of the TDI dose, the gap between hepatocytes was widened, mitochondria were swollen and vacuolated, and mitochondrial cristae disappeared. The expression levels of HO-1 gene and protein in the liver tissues of the low, medium, and high dose groups were significantly higher than those in the control group (P<0.05). Compared with the control group, the number of HO-1 positive cells in the low, medium and high dose groups increased and the staining increased gradually, and the difference was statistically significant (P<0.05). Conclusion TDI exposure can cause oxidative damage to rat liver tissues and induce the expression levels of HO-1 gene and protein expression.
