The Effect of Silver Diamine Fluoride on Salivary Biofilm

Author: Meekyung SEO ¹ ; Ji-Soo SONG ; Teo Jeon SHIN ; Hong-Keun HYUN ; Jung-Wook KIM ; Ki-Taeg JANG ; Sang-Hoon LEE ; Young-Jae KIM
Author Information

1. Department of Pediatric Dentistry, School of Dentistry, Seoul National University
Publication Type:1
From: Journal of Korean Academy of Pediatric Dentistry 2020;47(4):406-415
CountryRepublic of Korea
Language:Korean
Abstract: Silver diamine fluoride (SDF) is an effective and efficient agent for arresting dental caries. It can be useful in treating children with behavioral or medical limitations. The purpose of this study was to evaluate the antimicrobial effect of SDF by using salivary biofilm.
Pellicle-like saliva coated structure was prepared by using unstimulated saliva. For developing cariogenic biofilm, Streptococcus mutans was added to the mixture of pooled saliva and inoculated into a saliva coated glass or chamber. SDF was applied to cariogenic biofilm to evaluate the antimicrobial effect of SDF. As time passed, total bacteria and S. mutans were reduced after application of SDF (p < 0.000). Confocal laser scanning microscope also showed the increment of the ratio of dead cell.
As a result of experiment using enamel and dentin of primary teeth, it was confirmed that the growth of cariogenic biofilm was inhibited when the SDF was treated (p = 0.029 each).This study showed excellent anti-microbial effect of SDF. And anti-caries effect in clinical practice can be expected.