Antimicrobial Effect of Photodynamic Therapy Using Plaque Disclosing Agent
10.5933/JKAPD.2020.47.2.120
- Author:
Junhee KHO
1
;
Howon PARK
;
Juhyun LEE
;
Hyunwoo SEO
;
Siyoung LEE
Author Information
1. Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University
- Publication Type:1
- From:
Journal of Korean Academy of Pediatric Dentistry
2020;47(2):120-127
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) using plaque disclosing agent, 10 - 20 mM erythrosine, as a photosensitizer.
Multispecies cariogenic biofilms containing Streptococcus mutans , Lactobacillus casei and Candida albicans were formed on hydroxyapatite disc. 20 μM, 10 mM and 20 mM erythrosine were applied as a photosensitizer for 3 minutes, and then light-emitting diode (LED) irradiated for 24 seconds. Colony-forming unit (CFU) were measured and biofilms were observed using confocal laser scanning microscopy (CLSM). CFU were significantly decreased in the PDT groups using 10 - 20 mM erythrosine (10 mM, 20mM) and the results were also confirmed by CLSM.
This study confirms the high antimicrobial effect of photodynamic therapy using plaque disclosing agent as a photosensitizer.
