Evaluation of repetitive sequence-based genomic fingerprinting for molecular classification and identification of vibrio species.
- Author:
Gyu Won KIM
1
;
Hun Jong CHUNG
;
Chul Min PARK
;
Kijeong KIM
;
Wonyong KIM
;
Sang In CHUNG
Author Information
1. Department of Microbiology Chung-Ang University College of Medicine1 Seoul, Korea. sichung@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Vibrio species;
Vibrio vulnificus;
rep-PCR genomic fingerprinting
- MeSH:
Classification*;
Dermatoglyphics*;
Vibrio vulnificus;
Vibrio*
- From:Korean Journal of Medicine
2006;71(2):189-197
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The aims of this study were to compare the suitability of repetitive-PCR genomic fingerprinting procedures to investigate genetic relatedness of the genus Vibrio and its applicability for the molecular identification of Vibrio vulnificus. METHODS: Forty-eight Vibrio strains were included for this study. REP-, ERIC-, BOX- and SERE-PCR were compared with 13 members of the genus Vibrio. RESULTS: REP-, BOX- and SERE-PCR showed V. vulnificus strains could not be separated well from other Vibrio species. However, approximately 320 bp of highly discriminatory specific fragments was recovered from V. vulnificus strains by ERIC-PCR. CONCLUSIONS: ERIC-PCR could be used as rapid classification and identification methods of V. vulnificus from other members of the genus Vibrio.
