Expression and prognostic significance of microRNAs in Korean patients with myelodysplastic syndrome
- Author:
Yunsuk CHOI
1
;
Eun Hye HUR
;
Ju Hyun MOON
;
Bon Kwan GOO
;
Dae Ro CHOI
;
Je Hwan LEE
Author Information
- Publication Type:Original Article
- From:The Korean Journal of Internal Medicine 2019;34(2):390-400
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/AIMS:Various alterations of microRNA (miRNA) expression have been reported in myelodysplastic syndrome (MDS). We aimed to investigate the unique patterns and prognostic significance of miRNA expression in Korean patients with MDS.
METHODS:Bone marrow mononuclear cells were collected from eight healthy controls and 26 patients with MDS, and miRNAs were isolated and assessed via quantitative real-time polymerase chain reaction for selected miRNAs, including miR-21, miR-124a, miR-126, miR-146b-5p, miR-155, miR-182, miR-200c, miR-342-5p, miR-708, and Let-7a.
RESULTS:MiR-124a, miR-155, miR-182, miR-200c, miR-342-5p, and Let-7a were significantly underexpressed in patients with MDS, compared to healthy controls. MiR-21, miR-126, 146b-5p, and miR-155 transcript levels were significantly lower in international prognostic scoring system lower (low and intermediate-1) risk MDS than in higher (intermediate-2 and high) risk MDS. Higher expression levels of miR-126 and miR-155 correlated with significantly shorter overall survival and leukemia-free survival. Higher miR-124a expression also tended to be related to shorter survivals.
CONCLUSIONS:Although our study was limited by the relatively small number of patients included, we identified several miRNAs associated with pathogenesis, leukemic transformation, and prognosis in MDS.
