Zinc Deficiency Elevates Fecal Protein, But Not Electrolyte and Short-Chain Fatty Acid, Levels in Enterotoxigenic Escherichia coli-Induced Diarrhea in Rats
10.5223/pghn.2022.25.1.79
- Author:
Ebuka E. DAVID
1
;
Muhammad A. YAMEEN
;
Ikechuku O. IGWENYI
;
Chidinma N. DAVID
;
Valentine NWOBODO
;
Akindele K. ISMAIL
Author Information
1. Department of Biochemistry, Alex Ekwueme Federal University Ndufu-Alike Ikwo, Ebonyi State, Nigeria
- Publication Type:Original Article
- From:Pediatric Gastroenterology, Hepatology & Nutrition
2022;25(1):79-86
- CountryRepublic of Korea
- Language:English
-
Abstract:
Purpose:To determine the effect of zinc deficiency on fecal protein, electrolyte, and shortchain fatty acid levels in both heat-stable (ST) and heat-labile (LT) enterotoxigenic Escherichia coli (ETEC)-induced diarrhea in rats.
Methods:Albino rats, weighing 100 to 150 g, were divided into 2 groups, with 15 animals each: non-zinc and zinc-deficient. These two groups were sub-divided into three sub-groups with five rats each: control (saline); LT-ETEC; and ST-ETEC. Sodium phytate (30 mmol/L) was added to the animals’ water to induce zinc deficiency, while diarrhea was induced using 5×10 9 ETEC cells/mL. Fecal protein levels were estimated using the Bradford method, while sodium and potassium levels were determined using atomic absorption spectrophotometry. Shortchain fatty acids were measured using gas chromatography-mass spectrometry.
Results:Among the non-zinc and zinc-deficient groups, there were significant increases (p=0.04), (p=0.03) in fecal protein concentrations (mg/mL) in the LT-ETEC- (4.50±0.33), (6.50±0.26) and ST-ETEC- (3.85±0.19), (5.98±0.32) induced groups compared to the control groups (2.60±0.52), (3.50±0.11) respectively. Fecal sodium and potassium levels (mg/L) were significantly (v=0.029) increased in non-zinc-deficient rats induced with LT-ETEC (9.35±0.95, 1.05±0.48), and ST-ETEC (9.96±1.02, 1.21±0.45) compared with the control group (8.07±0.44, 0.47±0.17) but the increase were not statistically significant (p=0.059) in the zinc deficient rat groups. Fecal acetate and propionate levels (mg/g) significantly (p=0.032) increased when induced with LT-ETEC and ST-ETEC in non-zinc and zinc-deficient groups compared with the control groups.
Conclusion:Zinc deficiency among rats with ETEC-induced diarrhea elevated fecal protein loss but may not have an effect on fecal sodium, potassium and short-chain fatty acid levels.
