Molecular Characterization of 170 New gDNA-SSR Markers for Genetic Diversity in Button Mushroom (Agaricus bisporus)
10.1080/12298093.2019.1667131
- Author:
Hyejin AN
1
;
Ick Hyun JO
;
Youn Lee OH
;
Kab Yeul JANG
;
Won Sik KONG
;
Jwa Kyung SUNG
;
Yoon Sup SO
;
Jong Wook CHUNG
Author Information
1. Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, Republic of Korea. jwchung73@chungbuk.ac.kr
- Publication Type:Note
- From:Mycobiology
2019;47(4):527-532
- CountryRepublic of Korea
- Language:English
-
Abstract:
We designed 170 new simple sequence repeat (SSR) markers based on the whole-genome sequence data of button mushroom (Agaricus bisporus), and selected 121 polymorphic markers. A total of 121 polymorphic markers, the average major allele frequency (M(AF)) and the average number of alleles (N(A)) were 0.50 and 5.47, respectively. The average number of genotypes (N(G)), observed heterozygosity (H(O)), expected heterozygosity (H(E)), and polymorphic information content (PIC) were 6.177, 0.227, 0.619, and 0.569, respectively. Pearson's correlation coefficient showed that M(AF) was negatively correlated with N(G) (−0.683), N(A) (−0.600), H(O) (−0.584), and PIC (−0.941). N(G), N(A), H(O), and PIC were positively correlated with other polymorphic parameters except for M(AF). UPGMA clustering showed that 26 A. bisporus accessions were classified into 3 groups, and each accession was differentiated. The 121 SSR markers should facilitate the use of molecular markers in button mushroom breeding and genetic studies.
