Metabolomics approach to serum biomarker for laxative effects of red Liriope platyphylla in loperamide-induced constipation of SD rats
10.1186/s42826-019-0009-x
- Author:
Ji Eun KIM
1
;
Young Ju LEE
;
Sung Ha RYU
;
Ji Won PARK
;
Mi Ju KANG
;
Hyeon Jun CHOI
;
Su Ji BAE
;
Yusang CHOI
;
Hyun Gu KANG
;
Kyu Bong KIM
;
Suhkmann KIM
;
Yong LIM
;
Dae Youn HWANG
Author Information
1. Department of Biomaterials Science, College of Natural Resources & Life Science / Industry Convergence Research Institute, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup Miryang-si, Gyeongsangnam-do 627-706, South Korea. dyhwang@pusan.ac.kr
- Publication Type:Original Article
- From:Laboratory Animal Research
2019;35(2):52-60
- CountryRepublic of Korea
- Language:English
-
Abstract:
Red Liriope platyphylla (RLP) is a known herbal medicine used in the treatment of some chronic diseases including constipation, neurodegenerative disorders, diabetes and obesity. To determine and characterize putative biomarkers that predict the laxative effects induced by RLP treatment, alteration of endogenous metabolites was measured in the serum of loperamide (Lop)-induced constipation rats after administration of RLP extract (EtRLP) using 1H nuclear magnetic resonance (1H NMR) spectral data. The urine volume and amounts, and weights and water contents of stools were significantly recovered in the Lop + EtRLP treated group as compared to the No group, whereas body weight and food intake maintained constant levels. Also, significant recoveries in the thickness of mucosa and muscle were detected in the colon of the Lop + EtRLP treated group. Furthermore, pattern recognition showed absolutely different clustering of the serum analysis parameters when comparing the Lop treated group and Lop + EtRLP treated group. Of the 33 endogenous metabolites, 7 amino acids (alanine, arginine, glutamate, glutamine, glycine, threonine and valine) and 8 endogenous metabolites (betaine, creatine, glucose, taurine, ethanol, lactate, glycerol and succinate) were dramatically increased in the Lop + EtRLP treated SD rats. These results provide the first evidence pertaining to metabolic changes in the constipation rats treated with Lop + EtRLP. Additionally, these findings correlate with changes observed in 15 metabolites during the laxative effects of EtRLP.
