Xylazole inhibits NO-cGMP pathway in fetal rat nerve cells
- Author:
Xinyu WANG
1
;
Yue WU
;
Lin LIU
;
Hui BAI
;
Zhiheng ZHANG
;
Mingchao ZHAO
;
Tianwen MA
;
Xiaopeng SONG
;
Lina JIA
;
Liangyu LV
;
Yue YU
;
Xinyu XU
;
Hong CHEN
;
Li GAO
Author Information
- Publication Type:Original Article
- From:Journal of Veterinary Science 2022;23(1):e16-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Xylazole (Xyl) is a veterinary anesthetic that is structurally and functionally similar to xylazine. However, the effects of Xyl in vitro remain unknown.
Objectives:This study aimed to investigate the anesthetic mechanism of Xyl using fetal rat nerve cells treated with Xyl.
Methods:Fetal rat nerve cells cultured for seven days were treated with 10, 20, 30, and 40 μg/ mL Xyl for 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, and 120 min. Variations of amino acid neurotransmitters (AANTs), Nitric oxide-Cyclic GMP (NO-cGMP) signaling pathway, and ATPase were evaluated.
Results:Xyl decreased the levels of cGMP and NO in nerve cells. Furthermore, Xyl affected the AANT content and Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase activity in nerve cells. These findings suggested that Xyl inhibited the NO-cGMP signaling pathway in nerve cells in vitro.
Conclusions:This study provided new evidence that the anesthetic and analgesic effects of Xyl are related to the inhibition of the NO-cGMP signaling pathway.
