Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China
- Author:
Ling ZHAO
1
;
Liang ZHANG
;
Xiaona SHI
;
Xujie DUAN
;
Huiping LI
;
Shuying LIU
Author Information
- Publication Type:Original Article
- From:Journal of Veterinary Science 2021;22(6):e66-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide.
Objectives:In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated.
Methods:Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis.
Results:A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%–86.8% and 67.7%–75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5).
Conclusions:The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.
