Anti-inflammatory effect of non-thermal atmospheric pressure plasma for periodontitis treatment: in vitro pilot study

Author: You li PARK ¹ ; Hyun-Joo KIM ; Ju-Youn LEE ; Sung-Hee JEONG ; Eun-Young KWON ; Ji-Young JOO
Author Information

1. Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University, Yangsan, Republic of Korea
Publication Type:Original Article
From:Journal of Dental Rehabilitation and Applied Science 2021;37(2):88-94
CountryRepublic of Korea
Language:English
Abstract: Purpose:The purpose of this study was to evaluate the anti-inflammatory effects of non-thermal atmospheric pressure plasma (NTP) on human gingival fibroblasts (HGFs) for clinical application of periodontal treatment.
Materials and Methods:HGFs were treated with Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS). Customized NTP device was developed for periodontal in vitro study. Cell viability was evaluated with cell counting kit-8. The levels of inflammatory cytokines, including interleukin (IL)-8 and 6, were determined by enzyme-linked immunosorbent assay.
Results:When NTP was applied, the cell viability did not change significantly, and there was no difference for 6 h and 24h. When Pg LPS was treated to HGFs, the secretion of IL-8 and IL-6 was increased compared to the control group. But when the NTP was applied, the secretion of them was significantly decreased.
Conclusion:NTP did not affect cell viability of HGFs. And it inhibited the LPS-induced production of IL-8 and IL-6.